Coding
Cse1

Part:BBa_K1218003:Design

Designed by: Trevor Kalkus, Gordon Wade, Alissa Greenberg   Group: iGEM13_Stanford-Brown   (2013-08-28)


CRISPR CasA E. coli (Modern)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to perform mutagenesis twice to remove PstI cut sites.


Source

E. coli DH5alpha genome

References